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生物素标记bcr基因探针的制备

Preparation of bcr gene probe labeled with biotin
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摘要 采用乙二胺化学修饰法制备生物素bcr基因探针,探针的检测敏感性达1pg,接近同位素标记探针的敏感性。该标记方法操作方便,标记效果稳定,探针可长期保存,试剂价格较低廉,为bcr基因探针的临床应用开辟了一条新途径。 Biotinylated bcr(breakpoint cluster region ) gene probe was prepared by a chemical labeling method of ethylenediamine modification procedure. The probe labeled by this method was able to detect lpg homologous DNA in dot hybridiration. The operation of the method was a convenience. The stable labeling efficacy, high detecting sensitivity of the probe. Low price of reagents and long term conservation of the probe make it a new route of the clinical application of bcr gene probe.
出处 《河南科技大学学报(医学版)》 1994年第3期160-162,共3页 Journal of Henan University of Science & Technology:Medical Science
关键词 bcr基因探针 生物素标记 CML bcr gene probe biotinylated CML
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