早孕期绒毛细胞短期培养的染色体脆性位点研究

A Study on Chromosomal Fragile Sites in Chorionic Villus Cells during Early Pregnancy.

筛选102份人工流产的绒毛标本,分组进行短期培养及染色体脆性位点(FS)的研究,结果表明:M199加MTX(终浓度4×10^(-8)mol/L)(2组)与单纯199(1组)比较,染色体畸变率(CAR)和脆性位点表达率(FR)差别均有显著意义(P<0.05);M199加Brdu(终浓度2×10^(-4)mol/L)(3组)与2组比较FR差别有极显著意义(P<0.01);RPMI1640加MTX(终浓度8×10-8mol/L)(4组)与2组比较中期分袭相数差别有极显著意义(P<0.05)。该项技术对探讨群体的遗传素质和对该群体的生活环境质量进行评价具有重要意义,为早孕期产前诊断提供了有效方法。

The Chromosomal fragile sites were studied in 102 samples of chorionic villi obtained induced abor-tion using different medium a and inducive.the find-ings were as follows: (l)significant diference(p<0. 05) between M199 plus MTX and single M199 in chromosomal aberratoon rate(CAR)and fragile site expression rate (FR);(2)a very significant differ-ence in FR; (p<0. 01)between M199 plus BrdU M199 MTX significant difference(p<0. 05)between RPMT1640 plus MTX and M199 plus MTX in metaphase chromosomal numbers. The results sug-gested RPMI1640 is suitable for short term culture of chorionic villus cells. MTX and BrDu are ideal in-ducers for chromosomal fragile sites of chorioni9c villus.