p53蛋白在鼻咽癌中的表达

P53 Expression in Nasopharyngeal Carcinoma

Ｐ５３基因突变是人类恶性肿瘤中最常发生的基因改变。Ｐ５３基因产物是一种核磷酸蛋白（Ｐ５３蛋白），该蛋白在肿瘤细胞中常有高表达。我们应用免疫组织化学ＳＰ法和Ｐ５３单克隆抗体（ＮＣＬ－ｐ５３－Ｄ０７）对有３年以上跟踪资料、用甲醛固定、石蜡包埋的５０例鼻咽癌（ＮＰＣ）组织切片进行Ｐ５３蛋白检测。结果显示：（１）Ｐ５３蛋白在ＮＰＣ细胞中为核染色阳性，正常上皮细胞的细胞核不着色；（２）４２例（８４％）阳性，其中强阳性１例，阳性８例，弱阳性３３例；阴性８例（１６％）；（３）Ｐ５３蛋白表达与否与ＮＰＣ的组织学类型、生物学行为无明显关系。

Mutation of the P5 3 gene is the commonest genetic event in human ma lignancy.The P53 gene encodes a unclear protein(P53 protein).Overexpression of the P53 protein is common in malignant cells. We used a monoclonol antibody NCLP53-D07 and a SP kit to detect the P53 protein of nasopharyngeal carcinomas(NPC)samples on formaldehyde fixed and paraffin sections. Of the 50 cases of NPC examined, 42(84%)showed positive staining, 8(16%)showed negative staining. The P53 expression was not correlated with the histological type and biologic behavior of NPC.