2－EHA促癌性及慢性二步致癌实验

2-EHA Promotion and 2-Stage Carcinogenesis Model

建立了Ｓｋｈ／ＨＲ－１裸鼠皮肤慢性二步致癌实验模型。实验鼠在一次涂抹发癌剂ＤＭＢＡ之后，连续１９周涂抹阳性促癌对照物（ＴＰＡ）及试验促癌物（２－ＥＨＡ），经过４０周实验观察，ＴＰＡ促癌作用得以复制；２－ＥＨＡ在１％，５％，１０％，２０％浓度范围内，表现明显的剂量－反应关系，各组动物平均患瘤数和患瘤率显著高于阴性对照组，在高剂量组，发生皮肤小瘤恶变为鳞状上皮癌。

Nude Skh/HR-1 mouse skin two-stage carcinogenesis modle was established in the experiment shown in the paper. After a single topical appplication of the initiator DMBA,mice were promoted with TPA as positive control or 2-EHA as experimental chemical for 19 weeks. At the end of experiment of 40 weeks,the promotion effect of TPA on the skin occurred as previously studies, the existence of adose-response relationship was shown within the range of 1%～20%concentrations.A statistically significant increase in papilloma yield and incidence occurred in 2-EHA-treated mice compared with those treated with acetone as solvent-promoted control.Mice treated with 10%and 20%2-EHA also developed squama carcinomas by 40 weeks post-initiation.