聚合酶链反应检测重型肝炎患者血清HBVDNA

DETECTION OF SERUM HEPATITIS B VIRUS DNA IN PATENTS WITH FULMINANT HEPATITS BY POLYMERASE CHAIN REACTION

采用ＰＣＲ法检测４８例重型肝炎患者血清中ＢＨＶＤＮＡ存在情况，并与斑点杂交检测进行比较，结果表明：ＰＣＲ检测３９例阳性，检出率为８１．３％（３９／４８）．ＨＢｓＡｇ（＋）、ＨＢｅＡｇ（＋）组ＰＣＲ检测均阳性（８／８），ＨＢｓＡｇ（＋）、ＨＢｅＡｇ（－）组阳性率为８８．０％（２２／２５），抗体阳性组检出率为７２．７％（８／１１），检测灵敏度为１．０ｆｇ；斑点杂交检测１５例阳性，阳性率３０．２％（１５／４８），各组斑点杂交阳性率均低于ＰＣＲ法．提示：（１）大多数重型肝炎患者血清中存在低滴度的病毒颗粒，对重肝发病有一定作用；（２）大部分ＨＢｅＡｇ阴性，抗体阳性的重型肝炎患者血清仍有传染性，应考虑抗病毒治疗．

eva from 48 fulminant hepatitis patients with hepatits B virus(HBV)were studied bypolymerase chain reaction(PCR)and dot-blot hybridization.Though the positive rate wasonly thicty peccent(15/48)by dot-bolt hybvidigation,HBV DNA could be detectde ineighty-one perecent(39/48)patients by PCR.all cases with hepatitis B surface antigen andantigen weve measuved to have seva HBV DNA by PCR;Whereas eighty-eight percent(22/25)patients who had seva HBsAg-positive and HBeAg-negative were also found to have HBVDNA by PCR. These data clearly suggest that most patients with severe hepatitis have lowseva HBV DNA that can not be detected by dot-blot hybrization.Antiviral therapy shoud beconsidered.