IEST、IFAT和Dot-ELISA诊断华枝睾吸虫病的比较研究

COMPARATIVE STUDIES ON THE DIAGNOSIS OF CLONORCHIASIS BY IEST,IFAT AND Dot-ELISA

用成虫冰冻切片抗原免疫酶染色试验(IEST)、间接免疫荧光抗体试验(IFAT)和斑点-ELISA(Dot-ELISA)对比检测了51例华枝睾吸虫病人血清,阳性率分别为92%、88%和92%.检测健康人血清50例,假阳性率分别为2%、4%和2%.用3法检测22例急性血吸虫病人血清、20例慢性血吸虫病人血清和15例肺吸虫感染者血清.IEST的交叉反应率分别为14%、5%和0;IFAT的交叉反应率分别为14%、10%和0;Dot-ELISA的交叉反应率分别为14%、5%和0.显示3种方法诊断华枝睾吸虫病均有较高的敏感性和特异性.而IEST和Dot-ELISA更适于现场应用.

Immunoenzymetic staining technique (IEST),indirect fluorescent antibody test (IFAT) with frozen sections of Clonorchis sinensis aduit worm and dot enzyme-linked immunosorbent assay (Dot-ELISA) were employed for examination in 51 casses with clonorchiasis and 50 healthy persons. The positive rate was 92% with BEST,88% with IFAT and 92% with Dot-ELISA, while the false rate was 2%,4% and 2% respectively. When 22 cases with acute schistosomiasis, 20 cases with chronic schistosomiasis and 15 cases with paragonimiasis were examined by IESA,IFAT and Dot-ELISA,the cross-reaction was 14%,5% and 0 with I-EST,14% ,10% and 0 with IFAT,and 14% ,5% and 0 with Dot-ELISA respectively. The results showed that IEST,IFAT and Dot-ELISA are effective methods of serological diagnosis of clonrchiasis,while IEST and Dot-ELISA are more suitable in the fields.