溶脲脲原体感染病人血清特异性IgM、IgG的测定

MEASUREMENT OF SPECIFIC IgG, IgM IN PATIENT'S SERA OF Uu INFECTION

在ＥＬＩＳＡ对特异性Ｕｕ抗体测定的基础上，以９０例Ｕｕ培养阳性者检测出抗Ｕｕ抗体８２例（９１．１％），其中Ｉｇ阳性６０例（６６，７％），Ｉｇ阳性４９例（５４．４％）；与３０例Ｕｕ多养阴性者比较，抗Ｕｕ抗体阳性４例（１３．３％），其中ＩｇＭ阳性１例（３．３％），ＩｇＧ阳性３例（１０．０％），差异显著（Ｐ＜０．０１）。ＥＬＩＳＡ测定血清抗Ｕｕ抗体的特异度为８７％，灵敏度９６％。该法是临床诊断Ｕｕ感染的又一有力手段，且对分析Ｕｕ感染的病程有重要参考价值。

Based on the establishment of ELISA technique, specific Uu antib0-dvwas detected in 82 cases from 90 Uu positive patient’s sera (91.9%)and 4 from 30 normal control(13.3%).Among the patients, specific lgM was 60/90 (66.97%)and specific IgG was 49l90 (54,4%).These lvere all significantly higher than that control(p<0.01).The sensitivity and the specificity of this ELIA technique were 96%and 87%respectively.ELSA not only can detect Uu infection but also is a useful method to differentiate the ill condition whether in aute or convalescent-phase.