摘要
A method for the determination of N-hydroxymethyl nicotinamide by spectrophotometry is described.N-hydroxymethyl nicotinamide was converted to acetate by acid-catalyzed acetylation,followed by base- catalyzed hydroxamation of the acetate ester and color development as the ferric hydroxamate chelate.The resulting colour solution was measured at 524nm,Beer's law is obeyed in the concentration range of 1--8x10^(-3) mol/L N- hydroxymethyl nicotinamide.
A method for the determination of N-hydroxymethyl nicotinamide by spectrophotometry is described.N-hydroxymethyl nicotinamide was converted to acetate by acid-catalyzed acetylation,followed by base- catalyzed hydroxamation of the acetate ester and color development as the ferric hydroxamate chelate.The resulting colour solution was measured at 524nm,Beer's law is obeyed in the concentration range of 1--8x10^(-3) mol/L N- hydroxymethyl nicotinamide.
