鸽血变虫(Haemoproteus columbae)配子发生时期的配子体和配子的超微结构

STUDIES ON THE FINE STRUCTURE OF GAMETOCYTE AND GAMETE OF HAEMOPROTEUS COLUMBAE IN THE STAGE OF GAMETOGENESIS

宿主红细胞内雌、雄配子体的表膜由外、中、内三层膜组成,但内层膜呈断裂状。雌雄配子体内含线粒体、色素颗粒、核糖核体、嗜饿体、内质网等胞器和胞核。宿主的细胞破裂后,雌、雄配子体瓣离胞外,外层膜亦同时破裂消失,故呈现二层膜结构。雄配子体分化之时,先见有部分胞质从内层膜断裂处伸出体外,形成亚铃形小体,随后被丢弃体外,胞核及主要胞器保留在原配子体内。核的四周出现4—6条轴丝;后渐移向胞质的四周。雄配子体分化早期,核内出现中心粒和纺锤丝。随后核膜消失,致密的染色质块散布于胞质之间,后渐移向轴丝附近。轴丝由"9+1"对微管组成。雄配子体出丝之前,先见轴丝从内层膜的断裂处伸出体表,形成鞭毛芽。随后每个染色质块带着部分胞质进入芽孢内。芽孢逐渐伸长,形成鞭毛状的雄配子。成熟的雄配子由核和轴丝两部分组成。游离的雌配子体分化时,先在核附近出现1—2条轴丝,同对胞核伸长和核内出现纺锤丝与中心粒,随后核分裂为二个成熟体,最后便形成雌配子。成熟雌配子的表膜由二层组成,内膜呈断裂,内含胞核、成熟体、胞口以及其它细胞器。

In the host cell, both macro and microgametocytes were surrounded by 3 distinct layer membranes, of which the innermost membrane revealed to be interrupted. They contained the ribosomes, mitochondria, en-doplasmic reticula, osmiophilic bodies and one nucleus in their cytoplasm, whereas the macrogametocyte had more ribosomes and osmiophilic bodies when compared with that of microgametocyte. Before the exflagellation of microgametocyte, a kinetosome appeared near the nucleus and was surround-ed by poorly defined electron dense material. When the host cell ruptured, the microgametocyte become extra—cellular, and its outermost membrane disappeared simultaneously. At that time, a part of cytoplasm pro-truded through the space of innermost membrane and formed as dumb—bell body which eventually threw a side. In the original microgametocyte, 4-6 axonemes were present arround the periphery of nucleus, and then removed to the periphery of cytoplasm. Soon the nuclear membrane disappeared and several chromatin masses were formed and migrated to the position mear axonemes. Each axoneme was composed of 9+2 mi-crolubules arranged as 9 pairs in the petipheral and 1 pair in the center. These axonemes extended to the sur-face and then grew into 4—6 flagellar buds. Each flagellar bud contained a chromatin mass together with a part of oytoplasm. Finally. these flagellar buds developed into 4—6 microgametes. The mature microgamete was thread—like in shape and composed of a single axoneme and a bend—shape nucleus. in general, the macrogametogenesis was slightly lagged behind the micro- gametogenesis. When macrogametoeyte was intracellular, its nuclens first prolonged its size and then divided in to 2 mature bodies.During the hosteell ruptured, its outermost membrane also disappeared at the same time, and then macroga-mete was formed. The macrogamete was surrounded by 2 layer membranes, and the innermost membrane ap-peared interrupted. The mature macrogamete was round in shape, containing a nucleus with double nuclear membrand, 2 atypical centrioles nearby nucleus, some ribosomes, 1—2 axonemes mitochondria, endoplasmic reticula and s number of pigments in its cytoplasm.