莼菜叶面微生物生态分布及产粘胶细菌5242菌株形成多糖发酵条件的研究

STUDIES ON ECOLOGICAL DISTRIBUTION OF MICROORGANISM ON THE SURFACE OF JUNSAI LEAVES AND ON FACTORS AFFECTING POLYSACCHARIDES FERMENTATION BY MUCILAGE-PRODUCING BACTERIA STRAIN 5242

作者调查了西湖莼菜生长的环境条件,分析了莼菜叶面微生物区系的生态分布;测定了莼菜多糖的组成分;对获得的一株产多糖高产菌株进行了初步鉴定;探索了该菌株产多糖的发酵条件和多糖发酵产物的多糖组分及生物学性质。结果表明:莼菜粘胶多糖中含有大量产胶细菌;产胶细菌发酵产物其多糖组成分与莼菜胶质多糖相似。证明了莼菜多糖的形成与产多糖细菌有着密切的关系。

Research work was focused on: Investigating of the environmental conditions for Junsai(Brasenia schrebet) growing; Analyzing the ecological distribution of microflora on the surface of Junsai leaves; Analyzing the constitutes of Junsai polysaccharides; Investigating the relationship existing between Junsai polysaccharides and polysaccharides-producing bacteria; Identifying the high-yield polysaccharides-producting strain; Studying on the factors affecting polysaccharides fermentation; Testing and measuring some physicochemical properties of polysaccharides produced by bacteria. The results are briefly described as follows: 1. Junsai polysaccharides contain a great deal of polysaccharides-producing bacteria, the distribution of polysaccharides-producing bacteria is related to some degree with Junsai polysaccharides. 2. The composition of Junsai polysaccharides is similar with that of polysaccharides produced by polysaccharides producing bacteria strain 5242. 3. The result of identification denotes that the polysaccharides producing strain 5242 belong to a new species of Acinetobacter, which the authors give the name A. brasenica n. sp. 4. As for polysaccharides fermentation, the polysaccharide product is largely accumulated during the late Logarithm phase and the Steady phase polysaccharides are being produced while the PH value is changing. 5. Strain 5242 has a high capability of producing polysaccharides and its extracellular polysaccharides has potential bioactivity.