红霉素高产株SIPI 2—13的推理选育和工业生产

RATIONAL SELECTION AND FERMENTATIVE PRODUCTION OF ERYTHROMYCIN PRODUCING STREPTOMYCES ERYTHREUS SIPI 2-13

本文对红霉素产生菌的推理选育和高产株的工业生产进行了研究。出发亲株86—610经紫外线诱变后,选得一些对红霉素前体丙酸抗性突变型菌株,所有这些菌株皆为正变株,其中一株SIPI 2—13比亲株增产34%。这一高产菌株的形态和生理特性与亲株不同;在工业生产中比原来的菌株14—74增产24%,最高水平达到7830u/ml;终产物的主要组分红霉素A在94%以上,效价为940u/mg。

Propionate kinase catalyzed propionate which is a key reaction in the biosynthetic pathway of erythromycin producing Streptomyces erythreus, and it is directly correlated with erythromycin productivity. Rational selection to propionate-resistant mutants could be obtained from S. erythreus 86-610 parent strain by UV irradiation and propionate induction. Erythromycin productivity of these resistant mutant strains was examinated. the result showed that the overproduction under the cultural condition of optimization have yielded by the all of seven mutant strains. One of them named SIPI 2-13 were more yield 34% than the parent strain 86-610 in flask and both differs greatly in morphological and biochemical characteristics. High yield strain SIPI 2-13 were more 24% than the production strain 14-74 in industrial fermentation, and the highest level about 7, 800u/ml were reached. The product of this mutant strain containigg erythromycin A above 94% and 940u/mg were obtained.