绿舒筋对艾氏腹水癌细胞DNA、RNA及蛋白质合成代谢的影响

Effects of EM on DNA, RNA and Protein Synthesis of EAC Cell

本文采用核素前体掺入法研究了绿舒筋对艾氏腹水癌(EAC)细胞DNA、RNA和蛋白质合成的影响。该药在0.1～5mg/ml范围内,不同程度地抑制~3H-TdR、~3H-UR和~3H-Leu掺入到EAC细胞的DNA、RNA和蛋白质中,呈浓度依赖性。去除绿舒筋后,~3H标记物掺入曲线呈递增型,与5-FUdR掺入曲线相似,提示绿舒筋是通过干扰癌细胞及蛋白质代谢发生抗癌作用的,而非损伤DNA模板药物。

By means of Liquid Scientillation Spectrometry, the effects of EM (Euonymusmup-inesis Loes et Rehd) on the incorporation of 3H-TdR, 3H-UR and 3H-Leu into EAC (Ehrlic ascites Cancer) were studied. The incorporation of 3H-TdR, 3H-UR and 3H-Leu into EAC cells were obviously inhibited after being exposed to EM, and the rate of inhibition showed a closed relationship to the doseage of the drug. But the inhibition recovered after the removal of EM,and the ascending curves of 3H-TdR,3H-UR,and 3H-Leu incorporation into DNA, RNA and Protein respectively were showed. The experiment suggested that EM exerted an inhibitory action on DNA synthesis in cancer cells through interfering the metabolism of DNA,which was similar with 5-FUdR's action. It was also proved that EM was a non-cytotoxicity drug that inhibited DNA synthesis under the experimental doses.