摘要
The sensitivity of PCR was determined for detection of HBV DNA in paraffin-embed-ded liver tissue with different methods for sample preparation.Of 8 cases of HBeAg-positiveHBV infection,HBV DNA was detected in 6 by extracting DNA from both frozen and dewaxedsamples,but in none by direct reaction.Of 12 cases subjected to Southern blot hybridization,HBV DNA was detected in 7 by this technique,in 10 by PCR with both methods of DNA extrac-tion and in 3 by direct PCR.The results showed that PCR was sensitive and was comparablewith blot hybridization in detecting intrahepatic HBV DNA.In comparison between differentmethods of sample preparation,the viral detection rate from the dewaxed samples was near thatfrom the frozen ones,while by the direct reaction HBV DNA could be detected only in a fewsamples with high level of infection.
The sensitivity of PCR was determined for detection of HBV DNA in paraffin-embed- ded liver tissue with different methods for sample preparation.Of 8 cases of HBeAg-positive HBV infection,HBV DNA was detected in 6 by extracting DNA from both frozen and dewaxed samples,but in none by direct reaction.Of 12 cases subjected to Southern blot hybridization, HBV DNA was detected in 7 by this technique,in 10 by PCR with both methods of DNA extrac- tion and in 3 by direct PCR.The results showed that PCR was sensitive and was comparable with blot hybridization in detecting intrahepatic HBV DNA.In comparison between different methods of sample preparation,the viral detection rate from the dewaxed samples was near that from the frozen ones,while by the direct reaction HBV DNA could be detected only in a few samples with high level of infection.
