摘要
In the present study,an anti-gastric cancer monoclonal antibody MGb<sub>2</sub>-daunomycin（DM）conjugate was prepared by cis-aconitic anhydride method.The distribution of the conjugatein nude mice bearing human gastric carcinoma SGC-7901 was investigated.Four to five moleculesof DM were introduced into each molecule of antibody while the antigen-binding capacity ofthe antibody was well-retained.This conjugate could be satisfactorily localized in the tumortissue.Ninety-six hours after intraperitoneal injection of <sup>125</sup>I-MGb<sub>2</sub>-DM conjugate,the ratio ofradioactivity per milligram of tumor to that per milligram of blood reached 203,the result beingquite similar to that of unmodified MGb<sub>2</sub>.
In the present study,an anti-gastric cancer monoclonal antibody MGb_2-daunomycin (DM)conjugate was prepared by cis-aconitic anhydride method.The distribution of the conjugate in nude mice bearing human gastric carcinoma SGC-7901 was investigated.Four to five molecules of DM were introduced into each molecule of antibody while the antigen-binding capacity of the antibody was well-retained.This conjugate could be satisfactorily localized in the tumor tissue.Ninety-six hours after intraperitoneal injection of ^(125)I-MGb_2-DM conjugate,the ratio of radioactivity per milligram of tumor to that per milligram of blood reached 203,the result being quite similar to that of unmodified MGb_2.
