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GENE CLONING OF HUMAN NERVE GROWTH FACTOR (hNGF) 被引量:1

GENE CLONING OF HUMAN NERVE GROWTH FACTOR (hNGF)
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摘要 NGF is one of the few endogenous neurotrophic factors which have been clarified in structure and gene sequence. The β subunit of NGF (β-NGF) has been proved to be effective in promoting normal development and differentiation of certain neurons in both central and peripheral nervous systemst. It has also shown to play
出处 《Chinese Science Bulletin》 SCIE EI CAS 1992年第13期1130-1133,共4页
关键词 NERVE growth factor POLYMERASE CHAIN reaction gene CLONING NERVE GROWTH FACTOR POLYMERASE CHAIN REACTION GENE CLONING
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  • 1Masafumi Nishizawa,Fumiko Ozawa,Takako Higashizaki,Keiko Hirai,Fumio Hishinuma.Biologically active human and mouse nerve growth factors secreted by the yeast Saccharomyces cerevisiae[J]. Applied Microbiology and Biotechnology . 1993 (5)
  • 2Levi-Montalcini R,Dal Toso R,della Valle F, et al.Update of the NGF saga. Journal of the Neurological Sciences . 1995
  • 3A. Ullrich,A. Gray,C. Berman,T. J. Dull.Human β-nerve growth factor gene sequence highly homologous to that of mouse. Nature . 1983
  • 4William S,Varon S,Peterson GM,et al.Continuous infusionof nerve growth factor prevents basal forebrain neuronal deathafter fim bria forniz transection. Proceedings of the National Academy of Sciences of the United States of America . 1986
  • 5Sambrook. J.Mol Cloning, A Laboratory Manual, 2nd ed. . 1989
  • 6Studier,W,Rosenberg,AM,Dunn,JJ,Dubendorff,JW.Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology . 1990
  • 7Becker,DM,Guarente,L.High efficiency electroporation. Methods in Enzymology . 1991
  • 8Negro A,Martini I,Bigon E,Cazzola F,Minozzi C,Skaper SD,Callegaro L.Synthesis of the biologically active beta-subunit ofhuman nerve growth factor in Escherichia coli. Gene . 1992
  • 9Warren S L,Fanger M,Neet L E.Inhibition of biological activity of mouse beta nerve growth factor by monoclonal antibody. Science . 1980
  • 10M Nishizawa,F Ozawa,T Higashizaki,K Hirai,F Hishinuma.Biologically active human and mouse nerve growth factors secreted by the yeast Saccharomyces cerevisiae. Applied Microbiology and Biotechnology . 1993

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