旱芹人工种子内培养物的形态发生和生理生化的研究

STUDIES ON MORPHOGENESIS AND PHYSIOLOGICAL CHANGES OF CULTIVATING TISSUES IN CELERY ARTIFICIAL SEEDS

本文讨论有关旱芹人工种子内部培养物由体细胞脱分化产生胚性细胞、胚状体,直到成株的过程中形态发生的问题,对胚性细胞所处的状态、胚状体筛选和畸形胚、胚性细胞(团)或子叶胚包裹人工种子及其萌发特点、播种基质等等方面进行了分析比较,并提出一些有关旱芹人工种子研制的基本结论。 实验对培养物的叶绿素含量和过氧化物同工酶谱进行了测定,并得到了水溶性蛋白和过氧化物酶的培养时间曲线,以期从生化指标探讨人工种子这一特定条件下培养物的生理生化变化。

The problems related to celery artificial seeds on morphogenesis of cultivating tissues in the process from dedifferenctiation of somatic cells, embryogenesis to plant formation were discussed.This paper also analysised state of embryonic cells, selection of embryoid, aberrant embryoid, coating and germination of embryonic cells/embryoid and medium for sown etc.Therefore, some fundamental conclusions of celery seeds preparation have been made.In order to probe into physiological changes in the special condition of synthetic seed, we tested isozymograms of perozidase and contents of chlorophyll in tissues. Moreover, dynamic curve of water dissolved protein and activity of peroxidase were obtained.