改良磺基水杨酸法显示GC谱带及两个新的GC变异型的发现

A MODIFIED METHOD FOR GC SUBTYPING AND THE DISCOVERY OF TWO NEW GC VARIANTS IN CHINESE PEOPLE

本文采用改良了Kühnl报告的磺基水杨酸沉淀法显示GC谱带的技术,显示了六种常见的GC亚型和GC变异型。并且采用该法发现了两个新的GC变异型基因Gc^(1c3)和Gc^(2c7),其基因频率分別为0.0008和0.0004。本法经济、快速,谱带清晰,可取代需抗GC血清的常规免疫固定技术。

An improved method for Ge phenotyping using sulfosalicylic acid staining after IEF is reported. Gc subtyping had been achieved and Gc variants were detected using this method without using anfi-Gc serum for immunofixation. Two new Gc variants Gc^(1c3) and Gc^(2c7) were discovered in Chinese people, and their frequencies were 0.0008 and 0.0004 respectively.