左旋吡喹酮对日本血吸虫成虫体表抗原暴露的影响

EFFECT OF LEVO-PRAZIQUANTEL ON THE EXPOSURE OF TEGUMENTAL SURFACE ANTIGEN OF SCHISTOSOMA JAPONICUM

感染日本血吸虫小鼠1次口服75mg/kg左旋吡喹酮或150mg/kg的混旋吡喹酮后,间隔10min～7d不同时间取虫,以IFA分别检测整体虫和成虫切片的体表抗原。结果显示,给药后10～30min有半数虫体体表有点状弱荧光;1～6h后虫体吸盘处和尾端有较明壳荧光;6h后,几乎整个虫体均有较强荧光。至第3d,虫体荧光强度较前减弱。且两治疗组虫体体表抗原暴露情况无明显差异。提示,左旋吡喹酮与混旋吡喹酮一样亦可干扰血吸虫成虫皮层代谢过程,使皮层损害,体表抗原暴露。

Mice infected with Schistosoma japonicum were treated with a single oral dose of levo-praziquantel 75mg/kg or racemic praziquantel 150mg/kg. 10min-7d after administration of the drugs, the infected mice were sacrificed and the parasites were studied. The tegumental antigen of the integral worms and of. the worm sections were tested by IFA. 10-30 min after treatment more than 50% of the tegumental surface of adult worm showed weak and small fluorescent spots in treated groups. 1-6h after treatment, the sucker and extremity of adult worm showed brighter fluorescent spots. From 6h after treatment, there were very bright fluorescent spots on the whole worm surface. 3d after treatment, the fluorescent intensity was weaker than before, and there was no significant difference between the levo-praziquantel group and racemic praziquantel group in the exposure of tegumental surface antigen. The results demonstrated that levo-praziquantel, just like the racemic-praziquantel, could disturb the tegumental metabolic course of the schistosomes, caused tegumental damage and exposure of adult worm surface antigen.