PVC-ELISA与5种常规方法对比检测血吸虫病血清抗体

COMPARATIVE STUDIES ON PVC-ELISA AND 5 KINDS OF ROUTINE SEROLOGICAL TESTS FOR DETERMINATION OF SCHISTOSOMAL ANTIBODIES

用PVC-ELISA检测急性血吸虫病人血清抗体,结果与粪检诊断符合率达97.27％,假阳性率为0.83％,交叉反应率为3.06％,未发现假阴性反应。通过本法与PS-ELISA、IDT、LAT、IHA和COPT等5种常规方法对比检测259份急性血吸虫病人血清,结果表明,PVC-ELISA的敏感性优于IDT、LAT、IHA村COPT,特异性与PS-ELISA和COPT无显著差异。

PVC-ELISA for determination of schistosomiasis antibodies was studied and compared with PS-ELISA, IDT, LAT, IHA and COPT. The positive rate of the PVC-ELISA for acute schistosomiasis was 97-27%, the false positive rate was 0. 83%, and the cross-reactive rate was 3. 06%. No false negative was found. The results of this paper showed that PVC-ELISA was better than other serological methods for the determination of schistosomal antibodies. The sensitivity and specificity of PVC-ELISA were similar with the commonly used ELISA , but the former was more economical, more simple, and easy to manage.