日本血吸虫Sj60-90KD抗原的保护性免疫研究

STUDIES ON THE PROTECTIVE IMMUNITY OF Sj 60-90 KD IMMUNOGEN IN SCHISTOSOMA JAPONICUM

从日本血吸虫可溶性虫卵抗原(SEA)中制备Sj60-90KD抗原,免疫C_(57)BL/6小鼠,通过ELISA检测抗体水平、脾淋巴细胞体外增生反应检测细胞免疫水平及其减虫率测定等评价其保护性免疫效果。结果表明,Sj60-90KD抗原免疫小鼠虽能产生较高水平的抗体,但未见明显保护效果,对小鼠ConA刺激的淋巴细胞增生反应似有抑制作用。

The 60-90 KD antigen of Schistosama japanicum isolated from SEA by SDS-PAGE was used to immunize female C57BL/6 mice viasubcutaneous injection route. Protective immunity against challenge infection with cercariae of S. japonicum was evaluated by the adult worm reduction rate, and the levels on humoral and cellular immunity in immunized mice. A high level of anti-SEA antibody litre in sera collected from immunized mice was detected by ELISA. The value of the adult worm reduction rate showed that there was no significant protective effect on challenge infection and no obvious difference beween immunized mice and non-immunized control groups. In addition, the data of T-cell proliferative response to ConA of spleen cells revealed that 60-90 KD antigen inhibited the response of T-cell to the mutagen. The possibility of induction of different results of immunity was discussed.