三种ELISA诊断血吸虫病价值的研究

STUDIES ON EVALUATION OF THREE KINDS OF ELISA FOR THE DIAGNOSIS OF SCHISTOSOMIASIS

自湖南、湖北、四川等血吸虫病流行区采集血吸虫病人血清343份和治愈者血清68份,非流行区正常人血清102份,华支睾吸虫病人和肺吸虫病人血清50份,用单盲法对用于血吸虫病诊断的经典ELISA、PVC-ELISA和FAST-SLISA进行了比较研究。经典ELISA、PVC-ELISA和FAST-ELISA的敏感性分别为97.08％,84.84％和100％,三法对流行区人群的特异性分别为63.24％,91.18％和33.82％,对非流行区人群和其它寄生虫病患者的特异性分别为100％,98.03％和95.04％,结果表明三种ELISA均有较高的敏感性和特异性,经典ELISA和FAST-ELISA适用于一般流行病学调查,而PVC-ELISA更适用于疗效考核以及控制地区的监测。

343 serum samples from patients with schistosomiasis (include acute, chronic, and late stage) and 68 samples from previously treated persons without eggs in their stool from endemic areas in Hunan. Hubei and Sichuan. 50 serum samples from patients with other parasitic diseases and 102 samples from normal persons in non-endemic areas were collected. The conventional ELISA, PVC-ELISA and FAST-ELISA were evaluated. All three tests showed high sensitivity and specificity. The sensitivity of conventional ELISA. PVC-ELISA and FAST-ELISA were 97. 08%, 84. 84% and 100% respectively. The specificity of conventional ELISA, PVC-ELISA and FAST-ELISA with serum samples of persons from endemic areas were 63. 24%, 91.18% and 33. 82%, while with serum samples of persons from non-endemic areas or with other parasitic infections were 100%, 98. 03% and 95. 39% respectively. The conventional ELISA and FAST-ELISA may be used for epidemiological surveys. The PVC-ELISA is useful for evaluation of ehemotherapy or for surveillance in the areas of control.