酶联免疫吸附试验诊断华支睾吸虫病初步观察

STUDY ON DIAGNOSIS OF CLONORCHIASIS WITH ELISA

实验感染兔取成虫制成1％华支睾吸虫成虫可溶性抗原。以1:1500包被ELISA板诊断华支睾吸虫病人血清,与粪检阳性符合率88.1％,健康人血清阴性符合率97.1％。用滤纸干血代替血清阳性符合率为87.0％,阴性符合率为85.4％,而在非流行区人群采滤纸干血阴性符合率则为95.1％。与丝虫病人血清未见交叉反应;与疟疾的交叉反应为3％;与血吸虫病的交叉反应为6.66％。结果表明应用ELISA诊断华支睾吸虫病是一种敏感性高、特异性强、重现性良好的免疫诊断方法,现场应用滤纸干血方法简便、微量,值得推广。

1 % soluble antigen of adult clonorchis from infected rabbit liver was used as 1 : 1 500 coating antigen in ELISA. Serum specimens from patients suffered from clonorchiasis and from normal healthy people in epidemic area as well as in non-epidemic area were taken and ELISA for antibody titers was performed. The results showed that the positive conformable rate to microscopic findings was 88. 1% in partients suffered from clonorchiasis while the negative conformable rate was 97.1 % in normal healthy people. When dry blood in filter paper from non-epidemic area people instead of serum was used for assay, the negative conformable rate was 95.1%. There is no cross reaction with sera from patients suffered from fi-laria, while the cross reactions with sera, from patients suffered from malaria and schistoso-miasis were 3% and 6. 66% respectively. The results show that ELISA is a simple, reproducible, sensitive and specific method in diagnosis of clonorchiasis.