麻风单克隆抗体的研究(Ⅳ):产生麻风单克隆抗体的基本条件探讨

Study on Monoclonal Antibody in Leprosy. (Ⅳ) Basic Conditions for Produce of Monoclonal Antibody

为产生抗麻风菌及其抗原成分的单克隆抗体,对小鼠免疫方案、饲养细胞的取舍、有关试剂的评价和影响腹水诱生的因素作了系统研究。作者认为在单抗产生过程中免疫小鼠时加分枝菌壁类佐剂及使免疫抗原决定簇暴露很重要;融合及克隆化时可不加饲养细胞;现在通用的几种1640差别不明显,但小牛血清的质量至关重要;在腹水产生的过程中诱导剂的性质、动物的性别及来源有明显影响,其它因素的影响须进一步研究。

For preparation of monoclonal antibody to M. leprae and its antigen component, schemes of immunizing mice, selections of feeding cells and relevant reagents and the factory influencing on produce of ascites have been systematically studied. The authors think that in the process of producing monoclonal antibody, for immunizing mice to add mycobacteritic wall adjuvant and to make the determinant of immunogens expose itself are very important. For fusing and cloning, feeding cells may not be added and differences among some existing 1640 are not significant, but the quality of calf serum is extremely important. The nature of inducers and sex and source of animal have significant influence on process of producing ascites, and the influences on it of other factors have to be further studied.