麻风菌的裸鼠足垫感染模型

Nude Mouse Foot Pad Model of Leprosy Infection

将得自治疗前和治疗后的LL及BL患者皮损处的细菌接种到Balb/c裸鼠右后足垫,又取裸鼠繁殖菌做亚代按种.18个月后回收菌达1000～2000倍,可见细菌已播散到未接种的足及舌、耳和鼻尖等处.接种一年后出现足垫肿胀。初代和亚代的足垫回收菌,其生长曲线大致相符.二株治疗后的麻风菌接种于裸鼠未见繁殖。从裸鼠组织中回收的抗酸茵经试管内培养和足垫平行对照,初步鉴定为人麻风菌。

Leprosy bacilli obtained from the skin lesions of LL and BL leprosy patients before and after treatment have been inoculated into the right rear foot pad of Balb/c nude mice and then the bacilli collected from the inoculated foot pad have been secondarily inoculated. The bacilli ricovered 18 months after inoculation showed an increase of 1000 to 2000 times in quantity and dissemination in the noninoculated feet, ears and apex of the nose. One year after the inoculation the foot pads have swoolen. The growth curves of the bacilli re- covered from primarily and secondarily inoculated mice are almost concordant. Two strains of leprosy bacilli obtained from treated patients had been inoc- ulated into the mice without growth. Through cul- ture in test tube and foot pad inoculation, the re- covered bacilli from nude mice tissues have been preliminarily identified as human leprosy bacilli.