赤豆酰脲含量变化的研究

Studies on the Changes of Ureide Content in Adzuki Bean (yigna angularis)

赤豆根瘤、叶片的酰脲含量发生日变化,而且根瘤或叶片尿囊素(ALN)含量和尿囊酸(ALC)含量日波动规律相似。叶片酰脲、尿囊素、尿囊酸含量日变化的明显高峰出现在16:00,比根瘤中明显的高峰迟1小时出现。根瘤尿囊酸含量高于尿囊素含量。Mn^(2+)(4mM)不影响种子萌发生长、幼苗酰脲含量以及尿囊素酶(ALNase)活力水平。Cu^(2+)(1—5mM)明显抑制种子萌发生长和尿囊素酶活力。KT(1—10ppm)和BA(1—25ppm)不影响幼苗尿囊素酶活力水平。ZT(1—25ppm)稍微影响幼苗酰脲含量。ABA(1ppm)抑制种子萌发生长,但未影响幼苗酰脲含量。

Changes of ureide, allantoin, allantoic acid content in leaves and nodules of adzuki bean (Vigna angularis) and effect of some exogenous compounds on the ureide content in seedlings were inverstigated. Diurnal variation of ureide content occured in leaves and nodules. Allantoic acid content was higher than that of allantoin in the nodules. Diurnal variation in ureide, allantoin, allantoic acid content in leaves displayed a peak at 16 : 00 which occured one hour later than that in the nodules. However, diurnal fluctuation patterns of allantoin content was similar to that of allantoic acid content in both the leaves and the nodules. Several compounds included in the growth medium were tested for their ability to affect the synthesis of allantoin, allantoic acid in germinating seedlings. Mn2+ showed no appreciable effect on the allantoinase activity and allantoin, allantioc acid synthesis, but Cu2+inhibited the allantoinase activity. However, KT (l-10ppm) and BA(l-25ppm) did not have any effect on the allantoinase activity. ZT(l-25ppm)had slight effect on the ureide content. ABA (lppm) repressed the germination but did not affect the ureide content.