人MNS_s血型系统抗N单克隆抗体的研制

Preparation and Characterization of Anti-N Monoclonal Antibody Against Human MNS_s Blood Group

应用杂交瘤技术,将 NS-1细胞与完整的红细胞免疫的 BALB/c 鼠脾细胞融合,获得MNS_s 血型系统分泌 IgG 抗 N 抗原的杂交瘤细胞株——10D_6。细胞培养上清效价1∶1024,亲和力7秒,经过8个月以上的连续传代培养,细胞增殖良好,性状稳定,持续分泌高效价,高亲和力,抗N 抗原的单克隆抗体。可作为血型检测的又一标准试剂。

One stable hybridoma cell line (10D_6) has been derived by fusing myeloma cell (NS-1) with spleen cells of mice immunized with intact N group red cells of human.The culture super-natant agglutinated N and MN red cells specifically with a titre of 1024 and avidity of 7 seconds.Thecells 10D_6 had been cultured over 8 months,secreting Anti-N IgG continuously.