应用ELISA间接法检测乙肝核心抗体

Establishment of Indirect ELISA Assay for Anti-HBc

作者应用高纯度基因重组的酵母菌生产的 HBcAg(YrHBcAg),建立了 ELISA 间接法。用酶标记抗人 Ig 检测结合于固相 YrHBcAg 上的抗-HBC。检测阳性血清的敏感性比竞争性抑制法高3个滴度;在36份参比血清的检测中,间接法和竞争性抑制法与 Abbott 试剂的符合率分别为72.1%和33.3%;在558份献血员血清的检测中,间接法比竞争性抑制法的检出率高7.7%。

This paper describes the highly purified Yeast recombinant HBcAg (YrHBcAg) used inan Indirect ELISA for anti-HBc.In indirect assay,binding of Anti-HBc to solid phase coated withYrHBcAg was detected with a Anti-human Ig enzyme conjugate.In contrast to competitive assay,theamount of labeled antibody bound to the solid phase was proportional to the level of anti-HBc in thepatient's serum/plasma.In addition to excellent sensitivity,this method showes a high specificity.Thesensitivity of this method was three times as much as competitive method in assay of anti-HBc positive serial serum.Based on all results in assay of 36 serum panel tested by Abbott Corzyme,the relative a-greement of indirect assay and competitive assay in comparison with Abbott Corzyme were 72.1% and33.3%,respectively.