摘要
本文报告用多聚酶链反应检测产生不耐热肠毒素大肠杆菌,使用二个寡核苷酸引物,直接从麦康凯平板的菌落中对该毒素的 A 亚基一段高度保守区域的 DNA 进行扩增,经琼脂糖凝胶电泳检测,30株细菌只有产生不耐热肠毒素大肠杆菌检测结果为阳性,其它细菌均为阴性.此法简便、特异、敏感.
This report described a polymerase chain reaction procedure for identification of heat-labile- toxin producing Escherichia Coli.Two oligonuclotido primers were used in this procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gone.Amplification was done directly on E.Coil colonies from Macconkey plates.Detection of the amplified product can be done by agarose gel electrophoresis.30 strains were detected.Only heat-labile-toxin-producting Eschericha Coli were showed positive result,the rest of the strains were presented negative result. This method is simple,specific,sensitive for routine diagnosis of this pathosen in clinical isolates.
出处
《医学研究生学报》
CAS
1992年第4期349-351,402,共4页
Journal of Medical Postgraduates
基金
全军"八五"医药卫生科研基金