用多聚酶链反应检测LT-大肠杆菌

Detection of heat-labile-toxin of enterotoxigenic Eschericha Coli by polymerase chain reaction

本文报告用多聚酶链反应检测产生不耐热肠毒素大肠杆菌,使用二个寡核苷酸引物,直接从麦康凯平板的菌落中对该毒素的 A 亚基一段高度保守区域的 DNA 进行扩增,经琼脂糖凝胶电泳检测,30株细菌只有产生不耐热肠毒素大肠杆菌检测结果为阳性,其它细菌均为阴性.此法简便、特异、敏感.

This report described a polymerase chain reaction procedure for identification of heat-labile- toxin producing Escherichia Coli.Two oligonuclotido primers were used in this procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gone.Amplification was done directly on E.Coil colonies from Macconkey plates.Detection of the amplified product can be done by agarose gel electrophoresis.30 strains were detected.Only heat-labile-toxin-producting Eschericha Coli were showed positive result,the rest of the strains were presented negative result. This method is simple,specific,sensitive for routine diagnosis of this pathosen in clinical isolates.