三类Ig抗甲状腺球蛋白独特型抗体的检测与意义

The determination of 3 class-specific antithyroglobulin antiidiotypic antibody and its clinical significance

用兔抗人甲状腺球蛋白(ATG)包被,建立了检测 IgG 类、IgA 类、IgM 类抗 TG 独特型抗体(ATG—Ab_2)的方法。此法可不受类风湿因子和 TG 与抗 TG 免疫复合物(TG—IC)的干扰.以正常对照组 A 值的(?)+3 s 为正常上限,甲亢、桥本甲状腺炎、甲减患者的 ATG—Ab_2总阳性率分别为58.2%、100.0%与40.0%.三类 Ig 的 ATG—Ab_2均存在.对甲亢患者检测的结果表明,ATG—Ab_2与 ATG 相互间具有排他性,提示前者对 ATG 的产生可能有负调节作用.

The microtitration plates were coated with rabbit anti-hTG antibody and indirected ELISA was established to assayIgG,IgA,IgM class- specific antithyroglobulin antiidiotypic antibody (ATG-Ab2).This method was not interfered with RF and TG-IG.The upper limit of nomal is (?) 3s of nomal subjects absorbence.The total positive rate of ATG-Ab2 in sera of the patients with Grave's disease,Hashimoto disease and Hypothyroidism were 58.2%,100%,40.0%,respectively.3 class-specific ATG-Ab2 were all e isted in patient's sera.The assaying results of Gravers disease indica- ted that it may be down regulation effect of ATG-Ab2 on ATG.