青霉素致金黄色葡萄球菌及表皮葡萄球菌L型变异的实验研究

A Laboratory Study of L-variation of Staphylococcus Aureus and Staphylococcus Epidemidis Induced by Penicillin

本文在实验室条件下观察了青霉素致金黄色葡萄球菌和表皮葡萄球菌L型变异的作用及变异和返祖后两类细菌的生物学性状,对抗生素作用敏感性的差异。结果表明:高浓度的青霉素(6400u/ml以上)可诱导金黄色葡萄球菌变异为L型、低浓度青霉素(100～200u/ml)可致表皮葡萄球菌为L型、两菌变异株的生物学性状及对抗生素的敏感性很相似,但在不含青霉素的培养基中金黄色葡萄球菌L型较表皮葡萄球菌难彻底返祖。

The role of penicillin in inducing L-variation instaphy-Lococcus aureus(SA) and staphylococcusepidemidis(SE) was studied in the laboratory and dif-ference of bilolgical properties and sensitivity to anti-biotics of two kinds of bacteria, after variation andreversion, were observed respectively as well. The re-sults showed:Penicillin with higher concentration(>6400U/ml) could induce L-variation in SA whilepenicillin with lower concertration(100-200u/ml)did the same in SE; Both biological property andsen-sibiligy to antibiotics were similar between the va-riant strains of the two kinds of bacteria except for thedifference of reversion which was more difficult for SAthan for SE in the medium without pericillin.