催化分光光度法测定痕量硒

Determination of Trace Selenium by Catalysis Spectrophotometry

本文采用正交设计法选择了 Se^(4+)—Fe(EDTA)—NO_3^-催化系统的优化条件,建立了催化分光光度法测定痕量硒的方法。该法线性范围宽(0.03～0.70μg/32ml),选择性好,并有良好的精密度和准确度,检测限达3×10^(-10)g/ml,水样加标回收率为99.8～105.8%,变异系数为4.4～3.4%;尿样加标回收率为86.1～92.1%,变异系数为1.3～2.7%,适用于水质和尿样分析。

A method for determination of trace selenium has been developed,and the catalysis system of Se^(4+)—Fe(EDTA)— NO_3-has been optimized by orthogonal design method.The linear range of determination is 0.03～0.70μg/32ml.In Water the recovery of Se is 99.8～105.8% with relative standard deviation of 4.4～3.4%(n=5).in ur ine sample it's 86.1～ 92.1% with r·s·d.of 1.3～2.7%(n=5)The method is good selective and sensitive with a detection limit of 3×10^(-10)g/ml,and has been applied to natural water and urine sample with satisfactory results.