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DNA DIFFERENTIAL STAINING AND ITS CLINICAL APPLICATION

DNA DIFFERENTIAL STAINING AND ITS CLINICAL APPLICATION
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摘要 DNA differential stain is a simple method distinguishing cells of proliferative from quiescent stage. Double stranded DNA in quiescent cells is easily denatured by weak acid into single strand. As double stranded nucleic acid combined with methyl green and single stranded nucleic acid with pyronin, we make use of methyl green pyronin staining method to the cells treated with weak acid to distinguish proliferating from quiescent cells. This paper reports the observation of leukemia cells in the bone marrow smears of 100 cases of untreated acute leukemia by DNA differential staining method. The percentage of Go cells was lowest in ALL and highest in APL. DNA differential stain is a simple method distinguishing cells of proliferative from quiescent stage. Double stranded DNA in quiescent cells is easily denatured by weak acid into single strand. As double stranded nucleic acid combined with methyl green and single stranded nucleic acid with pyronin, we make use of methyl green pyronin staining method to the cells treated with weak acid to distinguish proliferating from quiescent cells. This paper reports the observation of leukemia cells in the bone marrow smears of 100 cases of untreated acute leukemia by DNA differential staining method. The percentage of Go cells was lowest in ALL and highest in APL.
出处 《Medical Bulletin of Shanghai Jiaotong University》 CAS 1992年第2期97-102,共6页 上海交通大学学报(医学英文版)
关键词 DNA DIFFERENTIAL stain METHYL green pyronin LEUKEMIA CELLS DNA differential stain methyl green pyronin leukemia cells
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参考文献8

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