摘要
Zone electrophoresis is suggested with flat capillary instead of circular ones to get over the disadvantages of both the narrow-bore and wlde-bore circular tubes (CT). This method was fully investigated in theory. A flat tube with a 2a×ka-mm (2a<ka) channel develops the maximum radial temperature gradient (π/k)-1 times less than an a -mm i. d. circular one. Compared with a 2a-ram i. d. circular tube, the flat one has at least a ((2k/π)-1)-fold increase in absorption response or decrease in detection limits, and the same fold increase in allowable sample throughput. The highest efficiency with a flat tube is thus expected. These were all tested in electrophoresis with 0.2×0.6-mm, 0.2-ram i. d. and 0.4-mm i. d. plastic tubes. Besides, the 0.2×0.6-mm flat tube required a less recovery time than the 0.2-mm i. d. tube and allowed a higher working voltage than the 0.4-mm i. d. tube, which implied that the flat tube offered the highest speed. The optimum k was observed at the values of 6—10 in the experiment and the recovery time was affected by the size of the tube and the viscosity of the solution.A preparation process of flat fluorocarbon polymer tubes is described in the paper, with a discussion on heat-deformatlon of the flattened tube.
Zone electrophoresis is suggested with flat capillary instead of circular ones to get over the disadvantages of both the narrow-bore and wlde-bore circular tubes (CT). This method was fully investigated in theory. A flat tube with a 2a×ka-mm (2a<ka) channel develops the maximum radial temperature gradient (π/k)-1 times less than an a -mm i. d. circular one. Compared with a 2a-ram i. d. circular tube, the flat one has at least a ((2k/π)-1)-fold increase in absorption response or decrease in detection limits, and the same fold increase in allowable sample throughput. The highest efficiency with a flat tube is thus expected. These were all tested in electrophoresis with 0.2×0.6-mm, 0.2-ram i. d. and 0.4-mm i. d. plastic tubes. Besides, the 0.2×0.6-mm flat tube required a less recovery time than the 0.2-mm i. d. tube and allowed a higher working voltage than the 0.4-mm i. d. tube, which implied that the flat tube offered the highest speed. The optimum k was observed at the values of 6—10 in the
