稀土元素铈铕钆铽在小鼠肝脏中的分布与沉积的电镜与X射线微区分析术研究

STUDY ON THE DISTRIBUTION AND DEPOSITION OF CERIUM, EUROPIUM, GADOLINIUM AND TERBIUM IN MOUSE LIVER WITH ELECTRON MICROSCOPY AND X RAY MICROANALYSIS

本实验分别给小鼠腹腔与静脉隔日交替注射稀土元素化合物氯化铈、氯化铕、硝酸钆、葡萄糖酸铽,共五次,总剂量为300～630mg/kg。电镜观察显示,四种元素均于枯否细胞和肝细胞中形成凝集体,枯否细胞中尤多。应用X射线微区分析术于凝集体分别探测到四种稀土元素的特征性X射线能谱峰。两种细胞的溶酶体内含较多高电子密度微粒。于胆小管腔亦发现高电子密度微粒群,提示肝细胞中的稀土元素可随胆汁排出。本文描述了四种稀土元素引起的肝脏形态学变化。

Four rare earth elements, cerium, europium, gadolinium and terbium, were given in the compound form respectively to mice in the intraperitoneal and intravenous way alternatively every other day for five times. The total doses were 300-530 mg/kg. The electron microscopic observation revealed that the four elements formed aggregates in both Kupffer's cells and hepatocytes. Characteristic X ray energy spectrum peaks of the four elements were positively detected on the aggregates separately by X ray microanalysis. Plenty of high electron dense particles were contained in the lysosomes of the two kinds of liver cells. Groups of high electron dense particles were found in the lumen of bile canaliculi, implying that the rare earth elements were excreted through bile. The morphological changes of liver caused by the four elements were described in this paper.