抗人红细胞凝集素血清的应用研究

APPLICATION OF ANTI-HUMAN RED BLOOD CELL AGGLUTININ SERUN

本文通过吸收抑制试验证明了抗人血红蛋白沉淀素血清中存在的那种能凝集人类红细胞的凝集素,实际上就是用人的红细胞免疫家兔而产生的抗人红细胞凝集素.为考察抗人红细胞凝集素血清对血痕种属的鉴识能力,本文用此抗血清作解离试验,对不同稀释度的新鲜人血痕、室内存放的久暂不一的人血痕和15种动物血痕进行了检测.得到的结果是,新鲜人血痕稀释至1/1000仍呈阳性;500例室内存放的人血痕中,存放时间在11年以下的448例全部能够准确测出;存放12-16年的52例人血痕中,有33例能够测出,余19例呈阴性;15种动物血痕全部呈阴性.

Absorption-inhibition test was carried out to prove that the agglutinin existing in anti-human hemoglobin precipitin serum is essentially anti-human red blood cell agglutinin. We used this agglutinin to identify the species of unknown bloodstains by elution test. The minimal identifiable amount of bloodstain corresponds to 0.001 ul of fresh blood; 448 cases of human bloodstain kept for less than 11 years showed correct reaction; 33 out of 52 cases of human bloodstain kept for 12～16 years showed positive result and the remaining 19 cases negative; all the fresh bloodstains of 15 species of animal showed negative result.