日本血吸虫保护性免疫机制的研究——活化巨噬细胞和超敏反应测定

STUDIES ON THE MECHANISM OF THE PROTECTIVE IMMUNITY AGAINST SCH1STOSOMA JAPONICUM-ANALYSIS OF MACROPHAGE ACTIVITY AND HYPERSENSITIVITY

本文用辐照致弱日本血吸虫童虫疫苗,冷冻辐照童虫苗和速冻致死童虫苗免疫雌性C57-BL/6小鼠。免疫后对小鼠腹腔巨噬细胞(MФ)的吞噬杀伤活性和超敏反应进行动态测定。结果表明2个活苗免疫组小鼠腹腔MФ体外表现出强烈杀伤童虫活性,杀伤力显著强于死苗免疫组和对照组(P<0.01);免疫后第6周杀伤力达峰值,第8周杀伤力下降;MФ杀伤力与保护力存在显著正相关(r=0.630,r>r0.05);活化MФ在免疫血清调理后杀伤活力有所增加,而免疫血清对于未活化MФ则无调理作用;活苗和死苗免疫鼠都出现强烈的速发型超敏反应,但与保护力之间缺乏相关性;迟发型超敏反应在本次实验中出现较弱。

Macrophage activity and hypersensitivity were evaluated in female C57BL/ 6 mice vaccinated with three Schistosomular vaccines. The results showed that in vivo the activated macrophages collected from live vaccine groups exhibited strong larvacidal activity. The cytotoxic activity of group Ⅰ & Ⅱ was significantly stronger than that of group Ⅲ & control group (P<0.01) . No significant difference was found bet-ween group Ⅲ and control group in killing activity of the macrophage. Larvacidal activity of group Ⅰ and group Ⅱ peaked at week 6 , began to decay at week 8 and had statistically significant correlation with resistance to chanllege. The immune serum opsonization enhanced the in vitro larvacidal activity of the activated macrophage from vaccinated mice. Macrophages of control or dead vaccine group had no activity of opsonization.Vaccinated mice developed significant immediate hypersen-sitivity and weak delayed hypersensitivity to soluble worm antigen preparation.