摘要
Populus tomentosa is one special species of poplar growing in North China. Mesophyllprotoplasts were isolated from the axenic shoots and cultured in the modified KM8p liquidmedium. Protoplast-derived cells started to divide after 7 days of culture. The frequencyof cell division reached about 20% in 10 days. The yellowish green calli grew compact andnodular after the hormone concentration of medium was adjusted. Shoot formation occurredwhen the protoplast--derived calli were transferred onto MS medium containing zeatin andIAA or NAA. The shoots rooted readily on 1/2 MS hormone--free medium.
Populus tomentosa is one special species of poplar growing in North China. Mesophyll
protoplasts were isolated from the axenic shoots and cultured in the modified KM8p liquid
medium. Protoplast-derived cells started to divide after 7 days of culture. The frequency
of cell division reached about 20% in 10 days. The yellowish green calli grew compact and
nodular after the hormone concentration of medium was adjusted. Shoot formation occurred
when the protoplast--derived calli were transferred onto MS medium containing zeatin and
IAA or NAA. The shoots rooted readily on 1/2 MS hormone--free medium.
基金
This research is granted by the National Biotechnology Programme.
