摘要
Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cellsfrom protoplasts divided to form somatic embryos directly. The somatic embryos grown to1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-mones.
Protoplasts were isolated from embryogenic calli derived from immature embryos of
wheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB medium
supplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cells
from protoplasts divided to form somatic embryos directly. The somatic embryos grown to
1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-
mones.
