摘要
An engineered E. coli strain containing high expression level of CT-B subunits has beenobtained by the application of recombinant DNA techniques. The B subunit can be secretedinto the medium and reaches 20- 40 μg/ml when this strain is incubated in a 50 1 fermenta-tion tank. The CT-B subunit purified with affinity chromatography in E. coli has the samecharacters as the natural CT- B subunit in molecular weight, N terminal amino acid analysisand antigenicity. The CT-B subunit has good immunogenicity and can be used as a preparation for protect-ing against diarrhea caused by V. cholera and enterotoxigenic E. coli. It can also be usedas a vector for hepatins.
An engineered E. coli strain containing high expression level of CT-B subunits has been
obtained by the application of recombinant DNA techniques. The B subunit can be secreted
into the medium and reaches 20- 40 μg/ml when this strain is incubated in a 50 1 fermenta-
tion tank. The CT-B subunit purified with affinity chromatography in E. coli has the same
characters as the natural CT- B subunit in molecular weight, N terminal amino acid analysis
and antigenicity.
The CT-B subunit has good immunogenicity and can be used as a preparation for protect-
ing against diarrhea caused by V. cholera and enterotoxigenic E. coli. It can also be used
as a vector for hepatins.
