抗湿热胶囊对热损伤大鼠β-内啡肽的影响

Study on the effect of Kangshire Capsule on β-endorphin in the rats with heat injury

目的 :通过研究抗湿热胶囊对热损伤大鼠 β -内啡肽 (β -EP)的影响 ,探究其抗热损伤的作用机制。 方法 :采用人工气候箱制成高温高湿环境 ,造成热损伤模型 ,测定大鼠下丘脑、垂体、血浆中 β -EP、的含量。 结果 :大鼠在受热后血浆和下丘脑中 β-EP的含量均升高 ,而垂体中 β -EP含量显著降低 ;给药组大鼠热损伤后血浆和下丘脑中 β -EP的含量高于模型组 (P <0 .0 5 )。结论 :抗湿热胶囊能明显提高热损伤大鼠血浆和下丘脑中 β -EP的含量 ,同时具有延缓垂体内 β -EP的水平下降的趋势 ,而高温反应中 ,β-EP能减轻热和疼痛刺激所致的生理反应 ,由此可以认为 ,抗湿热胶囊其机制可能是通过血浆和下丘脑中的 β -EP发挥作用 ,来提高机体的耐受力 。

Objective To study the effect of Kangshine Capsule on β-endorphin(β-EP)in rats with heat injury and the mechanism of Kangshire Capsule against heat injury.Methods The rat model of heat injury were created by artificial climate chamber and the level of β-EP in hypothalamus,pituitary and plasma were measured by radio immunoassay.Results The level of β-EP in hypothalamus and plasma of the rats with heat injury increased,while the level in pituitary decreased. In Kangshire Capsule group the level of β-EP in hypothalamus and plasma were higher than those in the model group( P <0.05).Conclusion Kangshire Capsule could enhance the resistance of the rats against heat injury by improving the level of β-EP in hypothalamus and plasma.