斑点免疫金银染色法(Dot-IGSS)诊断血吸虫病的初步研究

COMPARATIVE STUDIES OF DOT-IGSS AND DOT-ELISA IN DETECTING ANTIBODIES FROM SERA OF SCHISTOSOMIASIS PATIENTS

用斑点免疫金银染色法(Dot-IGSS)和斑点酶标法(Dot-ELISA)诊断日本血吸虫病37例,阳性率均为100％。检测正常人血清40份,Dot-IGSS法为阴性,Dot-ELISA法阳性4例(假阳性10％)。两种方法对37份血清不同稀释度的检测比较,经统计学处理p<0.001,差异显著,提示Dot-IGSS的敏感性和特异性均很高。且两法检测华支睾血清40份。均未出现交叉反应。

Dot-immunogold-silver Staining (Dot-IGSS) and Dot-ELISA were compared in detecting antibodies from sera of schistosomiasis patients. Soluble antigen was prepared with Schistosoma japonicum adult worms. Sera were collected from 37 schistosomiasis cases confirmed by fecal examination, while 40 sera from healthy individuals and 40 from clonorchiasis cases were tested simultaneously. The positive rates were recorded as 100% and 100% for these two methods respectively in schistosomiasis cases; false positive rates of 0 and 10% were exhibited in healthy controls, and none of the clonorchiasis cases show positive reaction.