摘要
Fast Plant(Brassica rapa,Cruciferae)leaf tissuefixed in glutaraldehyde-acrolein and post-fixed in os-mium,was examined for response to several easily-prepared heavy metal stains.Lead and uranium,separately and in combination,gave typical resultsacross the spectrum of cell organellets.As a single stainfollowing osmium,bismuth produced images seeminglyequivalent to lead and uranium.Phosphotungstic acidproduced very good membrane delineation but produceda washed-out background image similar to that from leadstaining.Carbohydrate compounds were especiallyresponsive to ruthenium;the cytoplasm and the matrixof all organelles were also stained very well.Theprocedures were no more demanding than traditionalstaining methods and may be easily used in research andteaching.Fast Plant materials are a reliable,quick andeasy source of living material.
Fast Plant (Brassica rapa, Oruciferae) leaf tissue fixed in glutaraldehyde-acrolein and post-fixed in osmium, was examined for response to several easily-prepared heavy metal stains. Lead and uranium, separately and in combination, gave typical results across the spectrum of cell organellets. As a single stain following osmium, bismuth produced images seemingly equivalent to lead and uranium. Phosphotungstic acid produced very good membrane delineation but produced a washed-out background image similar to that from lead staining. Carbohydrate compounds were especially responsive to ruthenium; the cytoplasm and the matrix of all organelles were also stained very well. The procedures were no more demanding than traditional staining methods and may be easily used in research and teaching. Past Plant materials are a reliable, quick and easy source of living material.
