移植胎肝细胞在体内分布和存活的示踪观察

Isotope-traced Observation of distribution and survival period of transplanted Fetal hepatocytes

我们观察了胎肝细胞的在体同位素标记及其影响因素;同时应用同位索标记的胎肝细胞,观察了胎肝细胞移植后在受体体内的分布格局和存活情况及其一些影响因素。实验发现,经胎鼠腹腔注射,^(125)I-UdR时,胎肝摄取^(125)I-UdR最多。标记胎肝细胞经外周静脉注射给成年小鼠后,主要分布在肺和肝中,这种分布格局受注射胎肝细胞数量和速度的影响。结果提示:在体法同位素标记的胎肝可用于胎肝细胞移植研究。

This paper presented the methods and the affecting factors of isotope labeleing of fetal hepatocytes in vivo. The results indicated that the fetal liver would contain a maximum of radioactivty if^(125)Ⅰ-UdR was injected into the abdominal cavity of the fetal mouse. Distributing patterns and survival period of the labeled fetal hepatoctes after transplantation into adult moue by some different routes had also been studied. It was found that the fetal hepatocytes were distributed mainly in the lung and liver by introvenous transplantation and the patterns were influenced by injecting speed and number of cells. It was concluded that these methods can provide some experimental basis for the clinical research of transplantation of fetal hepatoeytes.