摘要
We have cloned the E6 gene of human papillomavirus type 18 into anexpression plasmid pBD2.One of the recombinant plasmids (named pDV11) wasidentified by DNA analysis and protein product analysis.It could express a newprotein whose molecular weight correspods well with the expected one.Afterpurification,the expressed protein showed a positive result in countercurrentimmuno-electrophoresis with anti-β-gal serum and was proved to be the expectedβ-gal/E6 fusion protein.The physical map of pDV11 was also prepared.
We have cloned the E6 gene of human papillomavirus type 18 into an expression plasmid pBD2.One of the recombinant plasmids (named pDV11) was identified by DNA analysis and protein product analysis.It could express a new protein whose molecular weight correspods well with the expected one.After purification,the expressed protein showed a positive result in countercurrent immuno-electrophoresis with anti-β-gal serum and was proved to be the expected β-gal/E6 fusion protein.The physical map of pDV11 was also prepared.
基金
The project was supported by National Natural Science Foundation of China
