摘要
In the present study,daunomydn （DIM） was chosen to conjugate cowdently with amonoclonal antitibody,MGb<sub>2</sub>,against human gastric cancer cells via a cis-aconitic anhydride linker（directly） or a dextmn bridge （indirectly,） The mo-lar ratio of MGb<sub>2</sub> to DM in the conjugates was1:6 （direct method） and 1:54 （indirect method）,respectively.The ELISA results revealed theantibody after conjugation retained antigen-binding capacity.The conjugates showed a highly se-lective cytotoxicity to the target cells.In lh cytotoxidty test,cytotoxidty of the conjugates wasgreater than that of free DM or irrevalent conjugates to human gastric cancer cell lines,SGC-7901and very low as far as non-target cells （HeLa） were concerned.It is sugared that theselective cytotoxidty on target cells of the conjugates is mediated by the monoclonal antibody.
In the present study,daunomydn (DIM) was chosen to conjugate cowdently with a monoclonal antitibody,MGb_2,against human gastric cancer cells via a cis-aconitic anhydride linker (directly) or a dextmn bridge (indirectly,) The mo-lar ratio of MGb_2 to DM in the conjugates was 1:6 (direct method) and 1:54 (indirect method),respectively.The ELISA results revealed the antibody after conjugation retained antigen-binding capacity.The conjugates showed a highly se- lective cytotoxicity to the target cells.In lh cytotoxidty test,cytotoxidty of the conjugates was greater than that of free DM or irrevalent conjugates to human gastric cancer cell lines,SGC-7901 and very low as far as non-target cells (HeLa) were concerned.It is sugared that the selective cytotoxidty on target cells of the conjugates is mediated by the monoclonal antibody.
