大肠重度不典型增生的显微分光光度术研究

Microspectrophotometric study of the so-called severe dysplasia of the large intestine

采用显微分光光度计镜扫描法定量检测了61例正常结肠,重度不典型增生和大肠腺癌细胞核的 DNA 含量、核面积和平均吸收密度。结果表明:重度不典型增生细胞核的DNA 含量接近大肠癌,三组核面积之间差异显著(P<0.01),重度不典型增生和大肠癌的非整倍体检出率高且相近,极大部分重度不典型增生细胞已具有癌细胞的特征。研究发现:重度不典型增生实际上已处在癌的始发阶段,从而对大肠重度不典型增生的本质有了新的认识。

The DNA content,DNA distribution pattern and nuclear area were determined by microspectrophotometry with mirror scanning method in 61 cases of normal colon,the so-called severe dysplasia and large inte- stinal carcinoma.The results showed that nuclear DNA content of severe dysplasia was closely approached those of the large intestine(P>0.05). There is a significant difference in nuclear area between severe dysplasia, adenocarcinoma and normal colonic mucosa(P<0.01).We found DNA aneuploidy was significantly higher both in severe dysplasia and in large intestinal carcinoma.The study repored here suggested that most of the so- called severe dysplasia of the large intestine already gained definitive cha- racteristic of carcinoma in DNA content and distribution pattern.They were actually in an incipient phase of carcinoma.