全反式维甲酸对骨髓纤维细胞集落形成的影响

Effects of retinoic acid on the CFU-F from human bone marrow

本文主要通过纤维细胞集落的培养,观察了急性早幼粒细胞性白血病的骨髓纤维细胞集落,结果急性早幼粒细胞白血病的骨髓纤维细胞集落形成单位(CFU-F)较正常者明显低下4.3±4.9/2×~510细胞(n=9);完全缓解后,则上升为12.4±5.7/2×10~5(n=5)正常水平。体外试验结果显示维甲酸对 CFU-F 具有抑制作用。加入维甲酸之后,CFU-F 则降为3.11±3.5/2×10~5细胞,P【0.05。维甲酸诱导急性早幼粒细胞性白血病,外周血白细胞总数先出现高峰,然后进入低谷,后者可能与白血病细胞浸润有关外,还可能与骨髓纤维细胞被抑制有关.

We observed the CFU-F of 10 patients with acute promyelo- cytic leukemia (APL) and noted that the CFU-F in APL was 4.3±4.9/ 2×10~5 cells,much lower than that in the normal bone marrow specimens (P<0.05).When 5 patients achieved complete remission with the treatment of retinoic acid,the CFU-F became 12.4±5.7/2×10~5 cells,no obvious difference compared with the normal control(P>0.05).In vitro studies, when the culture system was added with 1μmol/L retinoic acid,the CFU-F decreased to 3.11±3.5/2×10~5 cells,lower than that in the control system(8.5± 3.5/2×10~5 cells,P<0.05).Our findings suggested that the growth of CFU- F of human bone marrow could be inhibited by retinoic acid.Perhaps these findings provided an evidence that retinoic acid would become an alternative approach of treatment of myelofibrosis.And perhaps the neutropenia after the peak of WBC was associated with the inhibited CFU-F during the RA- induced differentiation.