抗脊髓灰质炎病毒单克隆抗体试剂盒的研制及应用

Studies and Application of Diagnostic Kit with Monoctonal Antibodies for Detecting Poliovirus

本文从34株脊髓灰质炎病毒单克隆抗体中,挑选23株单克隆抗体(McAb)组成两套试剂盒:一套是具有各型毒株共同特异的单克隆抗体,偶联上异硫氰酸荧光素,用直接免疫荧光试验,对脊髓灰质炎病毒进行病原学诊断;一套是具有不同毒株特异的单克隆抗体,用中和试验或间接免疫荧光试验,根据抗体在抗原上识别表位的不同及其表位分布关系,可用于脊髓灰质炎病毒毒株问的差异和抗原变异的研究,以及疫苗株与野毒株的鉴别。近年来我们收集了286株脊髓灰质炎病毒标本,用上述试剂进行了病原学的诊断及抗原性质方面的分析研究。证明了这些试剂具有高度的特异性,可快速、简便、敏感地进行病原学诊断。应用单克隆抗体试剂从抗原表位水平来分析研究毒株的性质,鉴别疫苗相关株,这是一种较为理想的选择。

23 of 34 monoclonal antibodies (McAbs) to poliovirus have been scre-ened for making two panels of diagnostic kit.One panel made of 3 fluorescence-lablled McAbs was used for pathogenically identifying poliovirus.With the otherpanel it was possible to characterize the viruses,identify the intertypic different-iation of poliovirus strains,analyse the antigenic variation and recognize the va-ccine-related strains by immunfluorescence assay or microneutralization assay.In recent years,applying above methods,we have investigated 286 Poliovirusesstrains.The results have shown that the methods were specific,simple,rapid andsensitive for the diagnosis of polioviruses.The McAbs were excellent for analyz-ing topological relationships of antigenic epitopes and recognizing the vaccine-re-lated and/or wild strains.