轮状病毒低温传代后生物学特性的研究

Study on Biological Characteristics of Rotaviruses Passaged at Low Temperature

本文报道了动物轮状病毒(RV)SA_(11)及 NCDV 株经低温传代后生物学特性变化。这两个毒株分别属动物 RV 第3和第6血清型。本实验室用非洲缘猴肾细胞(BGMK)作为基质,将两株病毒连续传代,选择起始温度36℃,每传10代降低3℃,最终在29℃传20代,总传代数为50代。低温传代后的毒株,血凝活性明显下降,对组织培养感染性在39℃时,冷适应株低于原始株,血清中和试验表明:传代后毒株抗原性稳定,SA_(11)株具有良好的遗传稳定性。NCDV 株在传代过程中第5基因片段发生变异。

Two animal rotaviruse strains,Simian rotavirus SA11(serotype 3)and calf rotavirus NCDV(serotype 6)were pass,aged on BGMK cell(a africa green monkey kideny cell line)at 36℃,33℃ and 29℃ respectively Hemoagglutinin activities of the rotaviruses passaged at lower temperature decreased obviously.Infectivities of rotaviruses passaged at lower temperature in cell culture at 39℃ were lower than those of the original strains. The results of sero-neutralization test showed that after passages through 50 times,the two strains could still be neutralized by antibody to original strains.PAGE analysis indicated that SA11 remained their genetic stability throughout the study,while the fifth segment of the genome RNA of NCDV was changed.