抗急性非淋巴细胞白血病M_5型单克隆抗体研究

Research on M_5-type Monoclonal Antibody Against Acute non-Lymphocytic Leukemia

用急性非淋巴细胞白血病(急非淋)M_5型白血病相关抗原免疫 BALB/C 小鼠,取免疫鼠脾细胞与 NS-1骨髓瘤细胞融合,建立了2株能稳定分泌抗急非淋 M_5型白血病抗体的杂交瘤株。所分泌的单克隆抗体与急非淋 M_5型白血病有反应,与其他型白血病没有交叉反应,具有很好的特异性。CFU-GM 培养结果对骨髓造血干细胞没有影响。

BALB/C mice were immunized with the M_5-type associated antigen of acute non-lymphocytic leukemia,The spleen cells were fused with NS-1/1.Aq.4.1 m yeloma cells, and 2 cell strains which could secret anti-acute non-lymphocytic leukemia M_5-type monocl- onal antibodies were established.All the monoclonal antibodies secreted by these cell stra- ins gave positive reaction with the leukemia cells from acute non-lymphocytic leukemia M_2- type patient,but could not give any reaction with the cells from other type of leukemia patients.Themonoclonal antibodies produced by the cell strains showed good specificity.The result of CFu-GM culture did not affect myeloid stem cells.